sodium borate acid for DNA electrophoresis? - It's supposed to be much faster. Have you tried it? (Jun/28/2006 )

I used to use that buffer. It works very well . It’s cheaper, you can re-used many times. The resolution is not as good as with TAE, but it’s good enough. I ran gels at 250 or 300 V without any problem and used it for anything (purify bands for cloning, RFLP, anything). I don’t use it any more because of the rules of the company . I highly recommend it.

I used to use that buffer. It works very well . It’s cheaper, you can re-used many times. The resolution is not as good as with TAE, but it’s good enough. I ran gels at 250 or 300 V without any problem and used it for anything (purify bands for cloning, RFLP, anything). I don’t use it any more because of the rules of the company . I highly recommend it.

I've also found that the resolution is noticably better than TAE or TBE. Plus, this stuff is ridiculously cheap (the biotechniques paper actually compares the cost of this buffer to that of TAE or TBE). From my experience, however, I haven't been able to run my gels at the voltages described in the paper w/o melting the gel. Also, I have used SBA gel-purified fragments for ligations without problem.

Do a search for "sodium boric acid" and my username and you'll find a thread where the authors of that paper actually chimed in on this forum. PM me if you cannot find that thread and I'll try and post the link.

_hank

i was wondering what are the transfert buffer you use? and wat's the method compared to a standar gel?*
thanks in advance.