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GST fusion rotein - (Nov/28/2001 )

Hi, I tries to elute the GST-fusion rotein from the Glutathione beads. It does not work well for me, even though I used freshly made elution buffer. What is the alternative way to do that?


Hi Rui,

I'm using GST fusion protein as well and to elute the proteins from the Glutathion beads: Buffer: 10mM Tris/HCl pH 7.5, 50mM NaCl, 0.1mM EDTA, 1mM DTT, 5mM Glutathion. After adding the glutathion, readjust the pH to 8 with solid Tris. You can try to let the column sit in elution buffer 10 mins before collecting the fractions. Usually, I have a good rate of recuperation.