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K.lactis cruce extract - protein exptraction (Jun/26/2002 )

what is the best way to prepare K.lactis crude extract for portein purification. can it be processed the same way as S. cerevisiae?
thank you in advance for the help.


It depends on how you want to disrupt the cells. The easiest will probably be to crush them by vortexing motion with glass beads (simple: use vortex, advanced: use MSK cell homogeniser from B. Braun Biotech, Germany or similar). If the cells are about the size as yeast, try beads of about 0.45 mm diam.

Your advantage is that you avoid using enzymes.