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Wet transfer vs Semi-dry transfer - (Jun/03/2006 )

Which transfer system (wet or semi-dry) does your lab prefer use and why? What are the advantages/disadvantages of each system? ***Thanks your responses are greatly appreciated***


my lab uses a semi-dry transfer unit.

we like it because our transfers are faster and require a lot less buffer.

also, we use very large gels (non-standard) and we can fit them on the unit we purchased.


we use the semi dry transfert apparatus for reasons that mdfenko mentionned. But it allows also very good protein transfert overnight at 5V which wet transfert doesn't allow in our hands.