help: why PGK chosen as inner control? - (May/25/2006 )
why is PGK chosen as the inner control when detect the expression of some gene ?
is there any other gene can be inner control?
or PGK is just relatively express with some genes?
As PGK is a housekeeping enzyme (glycolytic pathway..) it should be expressed in all cells (more or less equally). Another possibility is GADPH (also glycolysis) or actin (cytoskeleton).
Personally I'd use rRNA as a internal standard, as it is the RNA with the most stable expression in my opinion.