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expression in Rosetta pLysS - how to get a good expression level?? (May/25/2006 )

I'm trying to express a toxic gene in E. coli. So I have to use pLysS strain to prevent basal expression and culture dead but the protein expression was very poor.
Has someone any tip to improve it??
I need almost 10mg of my protein urgently! sad.gif Help me please!! blink.gif


Is it a eukaryotic or membrane protein?

What exactly are you trying to express?