Silver Staining - (May/22/2006 )
I am supposed to remove a specific protein for my sample ~ 2 ul (0.7 ug/ul). How can I going about doing it? How much anitibody to add? How much protein A beads to add? I have repeat the experiment several times. I added 2 ul of antibodies and 1 ul of beads to 2ul of sample. But I am still unable to remove the protein based on the result I obtain from silver staining. Can anyone help me?
How do you know that the band that is staining with silver is your protein, and not another one of the same size? What does a western with antibody specific to your protein show?
I have the theoretical size of that protein. I have already done western blot to confirm that the particular protein is the one I wanted to remove.