# Dilution help - changing units (May/22/2006 )

Hello All:

Im sorry for this another topic regarding dilutions.. ive been through all the dilution topic in this fourm and non of them did actually help me...

anyway... lets suppose that i want to dilute 10uM to 1pmol in 100ul... i already knew the answer which is 10 uL, but want to be sure from where did it come and how.

i know we use the C1.V1=C2.V2

after changing the c1 unit to pmol , C1 = 10 000 000 pmol
applying the formula

V1= 1.100/10 000 000
V1= 0.00001
what is the final answer unit.. is uL, L or mL?

some said you have to differnt concentrations one is in M and the other is in mol..

thanks for looking...

nop
1 pmol/100 ul is equal to 0,01uM
so you have to make a dilution 1/1000 of your 10 uM stock to get 1 pmol/100ul (or 0,01uM)
if you want to use the formula, first define what volume you need
eg ... for a final V of 1 ml
CfVf=CoVo
Vo = 0,01uM * 1ml / 10 uM

you get 0,001 ml
or 1 ul
in others... a dilution 1/1000

-tertu-

1 M is 1 mol/L

-Missele-

Go to this site. It helps with calculations.

-scolix-