How to choose a suitable phage display system - (May/17/2006 )
Dear all:
I had no prior experience to this system, but am going to set up one in my lab and wondering if any expert could kindly offer some suggestions for newbie like myself.
We're interested in finding any possible short peptides, novel proteins, or antibody (all should be from the murine system) that can bind to a 29 kD leukocyte protein in rat we had recently purified. Can we achieve this by using phage display library? Or, if we should go for the yeast two hybrid system instead? All we desire to have is finding some protein that can bind to and possibly inhibit this 29-kD protein from interaction with its authentic ligand.
I'm really new to the research and would gratifully appreciate if anyone could help me get started on this project. Thanks in advance!!
I had no prior experience to this system, but am going to set up one in my lab and wondering if any expert could kindly offer some suggestions for newbie like myself.
We're interested in finding any possible short peptides, novel proteins, or antibody (all should be from the murine system) that can bind to a 29 kD leukocyte protein in rat we had recently purified. Can we achieve this by using phage display library? Or, if we should go for the yeast two hybrid system instead? All we desire to have is finding some protein that can bind to and possibly inhibit this 29-kD protein from interaction with its authentic ligand.
I'm really new to the research and would gratifully appreciate if anyone could help me get started on this project. Thanks in advance!!
There are many articals you can find which decribe how to find out inhibitors from phage libraries by phage display techniques. So first get the purified 29kDa protein and then use it as an antigen to screen the peptide library, antibody library, and thing you like.
Good lunk!