is it possible to extract DNA from filter paper by using autoclaved water? - DNA extraction from filter paper by water (May/10/2006 )
This is my first time also dealing with DNA impregnated sample. so i want to know, which is better, to elute with TE buffer or nuclease free ddH2O ? and what is the incubation temperature? is it in a fridge or RT?
TE is better. You should only have to elute for a few minutes. If you elute for a long time, I'd do it in the fridge. Transform 1 ul into 50 ul of cells and you should get plenty of colonies. Save the rest in the -80 if you want.