Neubaeur haemocytometer - (May/05/2006 )
Hi there
I have no idea how to do this question could someone please explain it-I have been told how to work it out....Calculate the volume of a square.Convert in Microliters. How many cells per square on average (divide number of cells by number of square) you then have a number of cells per volume and you convert in the right units
BUT! I still am not getting the right answer...would it be possible for some oneto write out quick steps on what I have to do
Using a Neubaeur haemocytometer, you make a count of leucocytes in a sample of human blood. In three 1 mm2 squares, you count 300 cells. What is your estimate of white blood cell density?(hint: the depth of the counting chamber is 0.1 mm).
-100 cells/µL
-9,000 cells/µL
-90 cells/µL
-900 cells/µL
-1,000 cells/µL
Thank you SO much
biology_06er
1000 cells / ul.
And what I do is to count cells in the 4 external squares and take the average per square. To the value u get divide by 0.1 since this are the cells in .1 ul volume.. In other words... multiply by ten and voila.. cells/ul
I have no idea how to do this question could someone please explain it-I have been told how to work it out....Calculate the volume of a square.Convert in Microliters. How many cells per square on average (divide number of cells by number of square) you then have a number of cells per volume and you convert in the right units
BUT! I still am not getting the right answer...would it be possible for some oneto write out quick steps on what I have to do
Using a Neubaeur haemocytometer, you make a count of leucocytes in a sample of human blood. In three 1 mm2 squares, you count 300 cells. What is your estimate of white blood cell density?(hint: the depth of the counting chamber is 0.1 mm).
-100 cells/µL
-9,000 cells/µL
-90 cells/µL
-900 cells/µL
-1,000 cells/µL
Thank you SO much
biology_06er
Hey there
Thanks heaps for the reply
so...I get the part where I have to find average cells per square...then I still dont get the next part (sorry for ignorance) i get that in 1uL=10^-6L but I'm still really confused! 
And what I do is to count cells in the 4 external squares and take the average per square. To the value u get divide by 0.1 since this are the cells in .1 ul volume.. In other words... multiply by ten and voila.. cells/ul
Hi there
I have no idea how to do this question could someone please explain it-I have been told how to work it out....Calculate the volume of a square.Convert in Microliters. How many cells per square on average (divide number of cells by number of square) you then have a number of cells per volume and you convert in the right units
BUT! I still am not getting the right answer...would it be possible for some oneto write out quick steps on what I have to do
Using a Neubaeur haemocytometer, you make a count of leucocytes in a sample of human blood. In three 1 mm2 squares, you count 300 cells. What is your estimate of white blood cell density?(hint: the depth of the counting chamber is 0.1 mm).
-100 cells/µL
-9,000 cells/µL
-90 cells/µL
-900 cells/µL
-1,000 cells/µL
Thank you SO much
biology_06er
Lets say you count 20 cells per square in 4 squares, then the average is 20 per square. so that is 20 cells per 0.1 ul that is 10(-7) L. if you divide 20 by 0.1 you get No. of cells per ul. that is 200 cells per ul or 200*10(6) cells per L .
Hope this is more explanatory
Thanks heaps for the reply
so...I get the part where I have to find average cells per square...then I still dont get the next part (sorry for ignorance) i get that in 1uL=10^-6L but I'm still really confused!
1000 cells / ul.
And what I do is to count cells in the 4 external squares and take the average per square. To the value u get divide by 0.1 since this are the cells in .1 ul volume.. In other words... multiply by ten and voila.. cells/ul
Hi there
I have no idea how to do this question could someone please explain it-I have been told how to work it out....Calculate the volume of a square.Convert in Microliters. How many cells per square on average (divide number of cells by number of square) you then have a number of cells per volume and you convert in the right units
BUT! I still am not getting the right answer...would it be possible for some oneto write out quick steps on what I have to do
Using a Neubaeur haemocytometer, you make a count of leucocytes in a sample of human blood. In three 1 mm2 squares, you count 300 cells. What is your estimate of white blood cell density?(hint: the depth of the counting chamber is 0.1 mm).
-100 cells/µL
-9,000 cells/µL
-90 cells/µL
-900 cells/µL
-1,000 cells/µL
Thank you SO much
biology_06er
ooo I get it now!!! Thanks Heaps tertu 
Hope this is more explanatory
Hey there
Thanks heaps for the reply
so...I get the part where I have to find average cells per square...then I still dont get the next part (sorry for ignorance) i get that in 1uL=10^-6L but I'm still really confused!
1000 cells / ul.
And what I do is to count cells in the 4 external squares and take the average per square. To the value u get divide by 0.1 since this are the cells in .1 ul volume.. In other words... multiply by ten and voila.. cells/ul
Hi there
I have no idea how to do this question could someone please explain it-I have been told how to work it out....Calculate the volume of a square.Convert in Microliters. How many cells per square on average (divide number of cells by number of square) you then have a number of cells per volume and you convert in the right units
BUT! I still am not getting the right answer...would it be possible for some oneto write out quick steps on what I have to do
Using a Neubaeur haemocytometer, you make a count of leucocytes in a sample of human blood. In three 1 mm2 squares, you count 300 cells. What is your estimate of white blood cell density?(hint: the depth of the counting chamber is 0.1 mm).
-100 cells/µL
-9,000 cells/µL
-90 cells/µL
-900 cells/µL
-1,000 cells/µL
Thank you SO much
biology_06er