# Neubaeur haemocytometer - (May/05/2006 )

Hi there

I have no idea how to do this question could someone please explain it-I have been told how to work it out....Calculate the volume of a square.Convert in Microliters. How many cells per square on average (divide number of cells by number of square) you then have a number of cells per volume and you convert in the right units

BUT! I still am not getting the right answer...would it be possible for some oneto write out quick steps on what I have to do

Using a Neubaeur haemocytometer, you make a count of leucocytes in a sample of human blood. In three 1 mm2 squares, you count 300 cells. What is your estimate of white blood cell density?(hint: the depth of the counting chamber is 0.1 mm).

-100 cells/µL
-9,000 cells/µL
-90 cells/µL
-900 cells/µL
-1,000 cells/µL

Thank you SO much
biology_06er

-biology_06er-

1000 cells / ul.
And what I do is to count cells in the 4 external squares and take the average per square. To the value u get divide by 0.1 since this are the cells in .1 ul volume.. In other words... multiply by ten and voila.. cells/ul

QUOTE (biology_06er @ May 5 2006, 02:08 PM)
Hi there

I have no idea how to do this question could someone please explain it-I have been told how to work it out....Calculate the volume of a square.Convert in Microliters. How many cells per square on average (divide number of cells by number of square) you then have a number of cells per volume and you convert in the right units

BUT! I still am not getting the right answer...would it be possible for some oneto write out quick steps on what I have to do

Using a Neubaeur haemocytometer, you make a count of leucocytes in a sample of human blood. In three 1 mm2 squares, you count 300 cells. What is your estimate of white blood cell density?(hint: the depth of the counting chamber is 0.1 mm).

-100 cells/µL
-9,000 cells/µL
-90 cells/µL
-900 cells/µL
-1,000 cells/µL

Thank you SO much
biology_06er

-tertu-

Hey there
so...I get the part where I have to find average cells per square...then I still dont get the next part (sorry for ignorance) i get that in 1uL=10^-6L but I'm still really confused!

QUOTE (tertu @ May 6 2006, 12:46 AM)
1000 cells / ul.
And what I do is to count cells in the 4 external squares and take the average per square. To the value u get divide by 0.1 since this are the cells in .1 ul volume.. In other words... multiply by ten and voila.. cells/ul

QUOTE (biology_06er @ May 5 2006, 02:08 PM)

Hi there

I have no idea how to do this question could someone please explain it-I have been told how to work it out....Calculate the volume of a square.Convert in Microliters. How many cells per square on average (divide number of cells by number of square) you then have a number of cells per volume and you convert in the right units

BUT! I still am not getting the right answer...would it be possible for some oneto write out quick steps on what I have to do

Using a Neubaeur haemocytometer, you make a count of leucocytes in a sample of human blood. In three 1 mm2 squares, you count 300 cells. What is your estimate of white blood cell density?(hint: the depth of the counting chamber is 0.1 mm).

-100 cells/µL
-9,000 cells/µL
-90 cells/µL
-900 cells/µL
-1,000 cells/µL

Thank you SO much
biology_06er

-biology_06er-

Lets say you count 20 cells per square in 4 squares, then the average is 20 per square. so that is 20 cells per 0.1 ul that is 10(-7) L. if you divide 20 by 0.1 you get No. of cells per ul. that is 200 cells per ul or 200*10(6) cells per L .
Hope this is more explanatory

QUOTE (biology_06er @ May 5 2006, 02:56 PM)
Hey there
so...I get the part where I have to find average cells per square...then I still dont get the next part (sorry for ignorance) i get that in 1uL=10^-6L but I'm still really confused!

QUOTE (tertu @ May 6 2006, 12:46 AM)

1000 cells / ul.
And what I do is to count cells in the 4 external squares and take the average per square. To the value u get divide by 0.1 since this are the cells in .1 ul volume.. In other words... multiply by ten and voila.. cells/ul

QUOTE (biology_06er @ May 5 2006, 02:08 PM)

Hi there

I have no idea how to do this question could someone please explain it-I have been told how to work it out....Calculate the volume of a square.Convert in Microliters. How many cells per square on average (divide number of cells by number of square) you then have a number of cells per volume and you convert in the right units

BUT! I still am not getting the right answer...would it be possible for some oneto write out quick steps on what I have to do

Using a Neubaeur haemocytometer, you make a count of leucocytes in a sample of human blood. In three 1 mm2 squares, you count 300 cells. What is your estimate of white blood cell density?(hint: the depth of the counting chamber is 0.1 mm).

-100 cells/µL
-9,000 cells/µL
-90 cells/µL
-900 cells/µL
-1,000 cells/µL

Thank you SO much
biology_06er

-tertu-

ooo I get it now!!! Thanks Heaps tertu

QUOTE (tertu @ May 7 2006, 11:13 PM)
Lets say you count 20 cells per square in 4 squares, then the average is 20 per square. so that is 20 cells per 0.1 ul that is 10(-7) L. if you divide 20 by 0.1 you get No. of cells per ul. that is 200 cells per ul or 200*10(6) cells per L .
Hope this is more explanatory

QUOTE (biology_06er @ May 5 2006, 02:56 PM)

Hey there
so...I get the part where I have to find average cells per square...then I still dont get the next part (sorry for ignorance) i get that in 1uL=10^-6L but I'm still really confused!

QUOTE (tertu @ May 6 2006, 12:46 AM)

1000 cells / ul.
And what I do is to count cells in the 4 external squares and take the average per square. To the value u get divide by 0.1 since this are the cells in .1 ul volume.. In other words... multiply by ten and voila.. cells/ul

QUOTE (biology_06er @ May 5 2006, 02:08 PM)

Hi there

I have no idea how to do this question could someone please explain it-I have been told how to work it out....Calculate the volume of a square.Convert in Microliters. How many cells per square on average (divide number of cells by number of square) you then have a number of cells per volume and you convert in the right units

BUT! I still am not getting the right answer...would it be possible for some oneto write out quick steps on what I have to do

Using a Neubaeur haemocytometer, you make a count of leucocytes in a sample of human blood. In three 1 mm2 squares, you count 300 cells. What is your estimate of white blood cell density?(hint: the depth of the counting chamber is 0.1 mm).

-100 cells/µL
-9,000 cells/µL
-90 cells/µL
-900 cells/µL
-1,000 cells/µL

Thank you SO much
biology_06er

-biology_06er-