Real time machines? - (May/05/2006 )

Hi,

Based on your experience how would you rate for realtime PCR the iQ5 from biorad and the 7500 of applied biosystems?

Thanks
Filipe

-filcastro-

We have two 7500 machines and they're fine (though we'd love a 7700). Software is good, reliability is good, support is good.

No idea about Biorad

-Doc_Martin-

What's the difference between the 7500 and 7700, if I may ask?

I personally use a 7700.

-Matt

-MisticMatt-

The 7700 is a physically bigger machine and can also accomodate a 384-well plate format. The 7500 runs a 96-well plate format and is therefore cheaper and provides less throughput.

-Doc_Martin-

Just FYI, the 7700 is discontinued and has been replaced by the 7900HT.

-soluene-

7700 - was based on " HIGH PERFORMANCE" non-peltier
cycler i.e PE9600
See Biotechiniques 1991

never used 384 plates
because John Atwood is teaching
that one-piece PCR plates are only " marginaly suitable"

New cyclers are simple cheap Peltier cyclers that were NEVER
cited as " high PERFORMANCE "

-artem-