Kit for anti-inflammatory cytokines measurement in serum - (Apr/26/2006 )
I would like to measure anti-inflammatory cytokines from human serum and was wondering if there is any commercially available kit for the basic panel of these cytokines (TNFalpha, IL-1, IL-6). Does anybody know something what would suit my purposes?
Thanks a lot for any help!
If you have a luminex machine you can do a luminex bead assay, this would allow you to multiplex all these cytokines at once, R&D Systems (www.rndsystems.com) have got pretty good luminex range for these cykokines
there r commercially available protein arrays, if u can take cytokine array system, by using small amounts of serum samples u can do western blotting. in a single experiment u can get lot of data, which can be realiable(coz they optimize the dilutions Ab n protien concentrations of cytokines ).
even u can make coustomized array, where u can give them list of cytokines in which ur interested, n they can give u membrane with those cytokines after optimization.
google by giving 'PROTEIN ARRAY+ cytokines"
hope this helps u
"If you have a luminometer you can do a luminex bead assay, this would allow you to multiplex all these cytokines at once, R&D Systems (www.rndsystems.com) have got pretty good luminex range for these cykokines"
I agree with Grapes' but it is expensive and not possible if you don't have access to a luminex machine (not luminometer which measures luminescence). For one or two cytokines, ELISA with matched pair antibodies from R&D or BD make work out cheaper.
Also there's a similar system to luminex from BD called CBA which can be measured on a flow cytometer or on a specialist machine.
All the best,