pEBB or pEBG vector maps - (Apr/26/2006 )
HELP! Does anyone have a map of the vector pEBB or know where I can find one. I am trying to use the pEBG vector and know how GST was cloned into pEBB but I have no info, like antibiotic resistance, on the parent vector pEBB. Thanks!
350 hits in Google for pEBB plasmid.
please google a little before...
Do some bibliography...
5' biblio gave me these refs :
pEBB is derived from pEF-BOS, an expression vector driven by the human elongation factor 1-a
promoter (35). pEBB has a multiple cloning site which replaces the BstXI-NotI stuffer fragment of pEF-BOS. pEBG is a glutathione S-transferase (GST) fusion vector derived by inserting a PCR-generated BglII-BamHI GST fragment from pGEX-2T (Pharmacia) into the BamHI site of pEBB. The entire coding region of Erk-1 cDNA was cloned into the BamHI site of pEBG as previously reported (49). pGDN-G consists of an SH3 domain-deleted abl gene which was constructed by PCR using wild-type murine c-abl cDNA and cloned into the pGDN retroviral expression vector as described previously (29); it is referred to in this work as DSH3-Abl. N17 H-Ras is a DN H-Ras allele (containing a serine-toasparagine mutation at position 17) expressed in pZIP-neo (6)
And SURPRISE !!!!!!!!!!!! Google reveals :
SEQUENCE and FEATURES of pEFBOS
so please let the rest of the work... but it's a pleasure to help