3 M sodium acetate preparation - (Apr/25/2006 )
Hi all, i have a doubt, when you prepare 3 M sodium acetate in certain volume the sodium acetate increase the volume for example, if i need to prepare 100 ml of this solution, i need to add 40.83g of NaC2H302 and then when i add 50 the water, the volume increase almost twice , so.... is necesary to add the rest of the water ??????, what is the real concentration de sodium acetate in this 50 ml of water that i add?????
The mw of sodiumacetat (without water) is 82,03 g/mol. For 100 ml of 3M solution you have to solve 3 * 82,03 * 0,1 = 24,609 g in a total volume of 100ml. Put the 24,609 g in a graduated cylinder, at some water for dissolving and than fill up for a total volume of 100ml!
For the concentration of your solution use
40,83 g / (82,03 g/mol * total volume in l of your solution), this is the concentration!!!
You just need to make it up to 100ml final volume rather than add 100ml to your solids
but when i add 50 ml odf water to the sodium acetate, in order to solubilize it, the solution volume is almost 100 ml so, i don't need to add the entire 50 ml of water left????
No you don't. Just add the little bit extra required to make 100ml final vol
You don´t have to add 100 or 50 ml. Just add enough water until a total volume of 100ml, composed out of the 24g salt and the rest water!
thank you so much!!!!, i did that but i was not complete sure about it.
Can this be autoclaved or do we need to filter sterilize?
u can autoclave
Strange - calculations done using concentration calculator show that you need about 87 mL of water to prepare such solution (exactly 86.74mL), much more than 50mL.