Problems in SDS PAGE - (Apr/18/2006 )
Hallo everybody,
I was running SDS electrophoresis today and after coomassie blue staining I had a clear gel (no bands and no marker). What can be the problem? The protein concentration is in order, the pH of the bufers I checked before running the gel and I used a new coomassie solutions. I was running the same samples as before. The protein was stored for 1 month in -20°C and for approximately three times thawed and freezed. The gel ran faster than usualy. I'm new in it and I don't know what happened:-(Thanks for your advices.
-A134-
QUOTE (A134 @ Apr 18 2006, 11:18 AM)
Hallo everybody,
I was running SDS electrophoresis today and after coomassie blue staining I had a clear gel (no bands and no marker). What can be the problem? The protein concentration is in order, the pH of the bufers I checked before running the gel and I used a new coomassie solutions. I was running the same samples as before. The protein was stored for 1 month in -20°C and for approximately three times thawed and freezed. The gel ran faster than usualy. I'm new in it and I don't know what happened:-(Thanks for your advices.
I was running SDS electrophoresis today and after coomassie blue staining I had a clear gel (no bands and no marker). What can be the problem? The protein concentration is in order, the pH of the bufers I checked before running the gel and I used a new coomassie solutions. I was running the same samples as before. The protein was stored for 1 month in -20°C and for approximately three times thawed and freezed. The gel ran faster than usualy. I'm new in it and I don't know what happened:-(Thanks for your advices.
Hi
It is probably that you have pored the stacking gel only.
-tan:-
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Hi
It is probably that you have pored the stacking gel only.
[/quote]
No I haven't....
-A134-