southern again - transfert (Apr/18/2006 )

hiok I read some protocol.
some day to do the transfet in 10xSSC overnight, or in 0,5MNaOH/1,5MNaCl orvernight or 0,5MNaOH/1,5MNaCl for 2H.
what is the best way

I have N+hybond nylon

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Prehydrate your membrane in 10XSSC
Transfert in 10XSSC overnight.

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thanks do you have a protocole?

before and after transfert

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go here

and scroll through till you find one appropriate for what you're working on and the tools at your disposal..

we often forget that this site started as a protocol-sharing tool and there's a great deal of valuable information on the 'other side' of POL

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you're right aimikins... The name is actually protocol-online...

for the protocol required ... : see http://www.jp.amershambiosciences.com/tech...xl_protocol.pdf
ps : hybond XL membranes have replaced the hybond N membranes, but should be treated equivalent

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