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Immunology - ELISA (Jun/13/2002 )

How to prepare TMB substrate solution that is stable for a month or so in the Lab


We use 10mg/mL of Methonal to make our TMB substrate, stored at room temperature in an amber vial.  We make up 10 mL's and use it until it runs out which is in about 2 months for us it still works great even at the end.  Hope this helps.