Protocol Online logo
Top : Forum Archives: : Molecular Cloning

problems in a double digestion with NheI and EcoRI - Cloning problem (Apr/06/2006 )

Pages: Previous 1 2 

Yeah, I used BSA when I used the Nhe Buffer 2 (last image posted)

I'll go ahead and try a two hour digestion and hopefully that'll make a difference

Thanks for all your help! smile.gif


Two hours did the trick.

You guys rock. biggrin.gif




thanks. cool.gif


Pages: Previous 1 2