anyone know the molecular weight of immunoglobulin heavy chain - (Apr/05/2006 )
I am wondering the molecular weight of immunoglobulin heavy chain when i perform CO-IP expriment,could anyone can help me ? any suggestion would be appreciated.
bobxiang
-bobxiang4-
under reducing conditions the heavy chain has a molecular weight of ~50kDa under non-reducing conditions the IgG immunoglobulin for example has a mw of 146-150kDa
-grapes of wrath-
and light chains are 25 kda under reduction
-Missele-
thanks grapes of wrath and Missele.
another question, do the immunoglobulin from mouse or rabbit have the same mw?
-bobxiang4-
slightly, yes
-Missele-
no problem, always glad to help
-grapes of wrath-