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In vitro transcription/translation with pDest14 - (Mar/30/2006 )

Has anyone used the Invitrogen Gateway pDest14 expression vector (with gene of interest inserted) with either the Invitrogen or Qiagen in vitro transcription/translation system? Any issues with protein yield if you have?

Any suggestions/comments would be much appreciated smile.gif


i didn't use the system you described, but you should keep in mind that the efficiency of in vitro translation depends a great deal, on your gen. that's why i'd say, to try is the only way to know wink.gif