BN/SDS 2D-PAGE - (Mar/30/2006 )
Do you have any suggestions on the composition of the buffer that need to be used to soak native gel before running a second dimension SDS-Page?
Now I am using normal running buffer for SDS-PAGE (TRIS/Glycine/SDS 0.1%) + DTT. I treat for half an hour.
you might want to try your normal sds sample buffer. the lower pH will help the proteins to stack and you will be adding tracking dye (you can use a lower concentration).