another PCR question - (Mar/26/2006 )

Although Pfu has a very low error rate it does not seem to give much yield especially if starting with very little DNA. Thats why many commercial PCR kits are mixtures of a proof reading enzyme and Taq. Taq amplifies but can not correct a mistake. The little bit of proof reading enzyme present is able to correct those mistakes (Taq probably stops or drops of when making a mistake before going on or what ever. mean while the mistakes often get corrected by the proof reading enzyme) Anyway, The error rate of such mixes are pretty low also and amplify much better then Pfu alone.
I know that people do this sometimes but i can't help you with the ins and outs but maybe somebody here does or you can find it on internet somewhere.
Sorry for not being of much help!

did you use the recommended buffer, cation, etc?
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yes I did
and I've heard that Pfu has twice longer time of elongation - and I also tried it
and then I tried mix with less amount of Pfu and more primers - and nothing
so now I'm using Taq, but maybe I will add some Pfu