Molecular biology with Bacillus subtilis - (Mar/25/2006 )
I am currently setting up Bacillus subtilis molecular biology and search for protocols to efficiently:
i. transform plasmids and ii. create knock-out strains.
Furthermore are there stable vectors for protein expression in Bacillus subtilis (best would be a shuttle vector usable for cloning in E.coli)?
Thanks to the helping hand out there.
Do you test the plasmid pHT3101
It has an replication origin in E coli and Bacillus.