vertical vs swinging ultracentrifugation - (Mar/20/2006 )

Hello there,

I have to perform a cellular fractionation, and the protocol I found in papers uses a vertical rotor, 0 degrees angle, like the VTi50 from Beckman. But the one I found, didn't work for x-reason.
So, I am thinking about using a swinging bucket rotor, as it is recommended when fractionating material that can be affected by the tubes walls as in fixed angle rotors.
The problem is that the centrifugal force will not be the same and I have to optimize the time.
I have been trying to read about the differential types of centrifugations but I couldn't find any reference where there is a correlation in time or force when changing from one rotor to another one, or considerations to take into account.

Do you think you can help me???? Any comment, is more than welcome!

Thanks, Marcfe

you can match g-forces for the 2 rotors.. beckman has an on-line calculator to do this:

http://www.beckmancoulter.com/resourcecent...s/rotorcalc.asp

if you can't attain the g-forces then you can match g*time.

Thanks!! I'll take a look there.