coulter counter - just checking something. (Mar/19/2006 )
I used a coulter counter to count my cells, and now i'm showing someone else, and they don't seem to get the dilutions (and i'm bad explaining dilutions).
So i would like if someone could check my math.
What i did was suspend my cells in 1mL of media, took 100uL of that, in 10mL of isoton. This gave me a 1:100 dilution. right? (100uL is 1:10 of 1mL.... which is 1:10 of 10mL. 10X10=100.)
What he did was suspend his cells in 100uL of media, and put that in 10mL of isoton. This would give him a 1:10 dilution... right?
Help? He doesn't believe me, and says he has a 1:100 dilution.
his is a 1:100 dilution of his preparation and yours is a 1:100 dilution of yours. yours started out 10 times the volume of his. if you wanted to equalize the samples then he would have to perform a 1:1000 dilution to match your sample. i would assume that your prep is your lab sop and he should get on board with that.
hope this helps.
that's not a dilution as he put all cels in his 10ml...