Role of BSA in restriction enzyme digestion - help (Mar/15/2006 )

Hi,would you please tell you why we add the BSA in the DNA digestion solution?
And the exact mechnism of the BSA to the enzyme.
Thank you very much.

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You may want to check out Promega Notes Magazine No. 59, 1996, pg. 46. Available online at http://www.promega.com/pnotes/59/5644h/5644h_core.pdf

In essence, it is said that the addition of BSA enhance enzyme performance by (a) providing additional protein which stabilize the enzyme ( balances any negative effects arising as a result of enzyme interaction with solid surfaces and/or the air/liquid interface.

All the best in your research!

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You may want to check out Promega Notes Magazine No. 59, 1996, pg. 46. Available online at http://www.promega.com/pnotes/59/5644h/5644h_core.pdf

In essence, it is said that the addition of BSA enhance enzyme performance by (a) providing additional protein which stabilize the enzyme ( balances any negative effects arising as a result of enzyme interaction with solid surfaces and/or the air/liquid interface.

All the best in your research!
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It is really very kind of you,Crybaby.Thank you very much.That article is very good too.
Thank you again.

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