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To find out the integration site of a transgene - (Mar/13/2006 )

Hello everyone
How could I find out the integration site of a transgene inside the genome ?
I am working on transgenic cotton varieties - its molecular characterization, structural and funtional stability analysis. I am trying to find out the adjoining gene sequences of a transgene inserted in to the cotton genome.




I read about vectorette PCR see here although this was in relation with one project that is frozen (at -70C...) right at this moment. And I haven't tryed it myself.


Maybe try a TAIL-PCR (Thermal assymetric interlaced PCR)? (it's normally performed for finding where T-DNA from agrobacterium has integrated, but probably can be adjusted for any integration).

Do a google search for "TAIL-PCR" or check out as a start.