Digoxigenin labeling ans substrate - (Mar/09/2006 )
For an in situ pcr , i am to do a dig labeling of the probe, I was thinking of pcr-dig labelling kit (Roche), but in the catalogue chemiluminiscence detection is recommended.
Can i go for colorimetric detection or should i use chemiluminiscence detection? Hope someone can give me a suggestion, Thank you in advance,
of course you can use colorimetric detection. it's just that chemiluminescence is more sensitive and can be stripped for reprobing.
Thank you very much for your reply