Protocol for the preparation of 20x Hank's Buffe - (Apr/10/2001 )

What is the protocol for the preparation of 20x Hank's Buffer (with Calcium)?

You mean Hanks balanced salt solution (HBSS)?

5.4mM KCl, 0.3mM Na2HPO4, 0.4mM KH2PO4, 4.2mM NaHCO3, 1.3mM CaCl2, 0.5mM MgCl2, 0.6mM MgSO4, 137mM NaCl, 5.6mM D-glucose, 0.02% phenol red (optional). Adjust pH to 7.4

Sorry, this is 1XHBBS