Inclusion Body Isolation - (Feb/27/2006 )

Is there easy to use and cheap method for isolation of inclusion bodies.
I donot want to purify proteins from isolation body.
I jsut want to isolate inclusion bodies for general lab experiments.
best regards

Well all you need to do is lyse your bacteria, centrifuge 13000rpm 20min, resuspend and lyse some more, centrifuge again, lyse again and centrifuge. You should end up with a witish sandy pellet with very little orangy bacteria.

Hope that helps

LeserattePD

I have to suspend bacterial culture in page buffer Tris Glycine or something else.
Or lysis should be with heatin at 95 centigarde or through sonification. what you recommend.
.
best regards
samita

Hi Samita,

why do you have to resuspend your bacteria in Tris Glycine? If you want only the inclusion bodies in Tris glycine then do a lysis on the bacteria and then resuspend the inclusion bodies in that buffer. Sonification work too.
LeserattePD