Plant leaf agroinfiltration method - (Feb/26/2006 )

I'm using the agroinfitration method to transiently express tagged proteins in N.benthamiana leaves. The Agrobacterium causes a microscopic damage to the cells by creating "bubbles" between the cells of injected leaf (like separating the walls of adjasent cells).
Did anybody encounter this problem, and any suggestions to avoide this?
thanks!
Raul

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hi
i had done agroinfiltration before , but never checked for bubbles.. usually it went smooth and got result ,, for example in transgenic plants with GFP, when the supperssor of gene silencing was intorduced thru agroinfiltration the suppression of gene silencing was seen,,,( by the green flourescence )

when u fill the needleless syringe with agroinfiltration mix , take care taht u fill it full and no air space between the solution and the tip that comes in contact with the leaf surface

hope it helps

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