Isolation of glycogen - cold precipitation, etc. (Feb/23/2006 )
I'll have my oral report on carbohydrates soon..
i've tried to research for further details on the mechanism of cold precipitation, the use of ethanol, and acetic acid for the isolation of glycogen from the supernatant fraction of liver (we used chicken liver, by the way)..
so can anyone help me on these?
a. what are the principles of using 'cold precipitation' in isolating glycogen?
b. what is the use of adding acetic acid to the supernatant solution (homogenized liver was centrifuged twice).
- according to the information i got, it was to release glycogen from the tissue. but i'm searching for a more a detailed explanation on this.
c. what is the use of adding cold ethanol?
- a discussion of a certain experiment in the web says that the purpose of EtOH is to precipitate glycogen. and it is via cold temperature to prevent enzymatic activity. Again, it would help me much if someone can provide a detailed discussion..
or, you may opt to suggest some websites that may be of help to my topic..
thank you very much!