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Beta gal assay - (Feb/20/2006 )

Hi ,
has anyone ever done a beta gal assay in yeast. I have been doing that for a week but every time i repeat it i get different results. My experiment are to stress the cells with salt and see how much of lacz gene is activtated.can someone help me with that .I am so tired and frusatated. which kit do u guys generally use . I am using the peirce kit.

Plz respond
Thanks in advance

sweety

-sweety-

someone reply

-sweety-