LiCl precipitation time - (Feb/07/2006 )
I use LiCl to precipitate RNA.
Precipitating overnight at -20°C gives me a larger RNA yield than 1 hour at 20°C.
A lot of protocols have short precipitation times. Ambion suggests 30 minutes.
What are your experiences with LiCl precipitation times?
Kind regards,
Jasper
-Jasper Bok-
Moderated:
I use LiCl to precipitate RNA.
Precipitating overnight at -20°C gives me a larger RNA yield than 1 hour at -20°C.
A lot of protocols have short precipitation times. Ambion suggests 30 minutes.
What are your experiences with LiCl precipitation times?
Kind regards,
Jasper
-Jasper Bok-