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LiCl precipitation time - (Feb/07/2006 )

I use LiCl to precipitate RNA.

Precipitating overnight at -20°C gives me a larger RNA yield than 1 hour at 20°C.
A lot of protocols have short precipitation times. Ambion suggests 30 minutes.

What are your experiences with LiCl precipitation times?

Kind regards,

Jasper

-Jasper Bok-

Moderated:

I use LiCl to precipitate RNA.

Precipitating overnight at -20°C gives me a larger RNA yield than 1 hour at -20°C.
A lot of protocols have short precipitation times. Ambion suggests 30 minutes.

What are your experiences with LiCl precipitation times?

Kind regards,

Jasper

-Jasper Bok-