SYBRSAFE staining - (Feb/04/2006 )

Hi,

We are thinking on switching to SYBRSAFE staining for visualizing PCR products. We would continue to use the UV translumninator though, as the invitrogen option to UV is quite expensive. Anyway, I was wondering if anyone has experience with this product, especially at setting up a digital camera to take pictures of the gels. Im looking for recommendations of what filters + Digital cameras to buy

thanks

hans

Are you really that concerned about the safety of EtBr? I'm pretty sure EtBr is cheaper...and from my experience, SYBRSAFE doesn't stain any better.

-Matt

In our experience it is about 2x less sensitive than EtBr. The filter also responds much more to the orange-G dye we use in our loading buffer, which makes it difficult to see very small fragments on the gel. We still are routinely using it, however.