SDS-PAGE - Trouble in running SDS-PAGE (Jun/02/2002 )

A handy calculator for making SDS-PAGE gels if you are still making your own:

http://www.changbioscience.com/calculator/sdspc.htm

Simply input the total volume, it will do all the calculations for you.

hi guys

could anyone help with a slightly unrelated problem: i get dreadful streaming on the gel (and western, therefore) with SDS lysates, which are absent on CHAPS and MPER lysates, all of breast cancer cell lines. would be grateful to know what protocol seems to give good clean lysate with SDS lysis buffer (re, specific steps such as sonication or heating etc).

cheers!
sean.

I think the protein concentration of your sample may be too high. Or is your sample in high salt concentration which can make the gel ugly?