centrifuge temperature - (Feb/01/2006 )
Does anyone know what makes the difference if we set the temperature to 4 C or RT or else during centrifugation?
-audrey-
It really depends on what you're spinning.
Generally, most protocols imply centrifugation at RT unless specifically stated.
-Hank
-haringsh-
While working with proteins we prefere 4C. At this temp you try to minimize degradation of your protein(s). So all our centrifuges are coled to 4C!
Oli
-ms-olli-
When for instance doing maxipreps during the isopropanol precipitation step I use the fuge @ 4°C because (as stated in the qiagen manual) this prevents overheating of the centrifuge (as this step takes 1 hour of spinning @ high speed).
-vairus-