Homemade RealTime Syb-GreenI-Mix - (Jan/27/2006 )

As KR suggested:
http://www.cif.ucr.edu/Assets/QPCR/QPCRmix.pdf

has a good report on it.

I use Applied Biosystems mater mix. As they need to label all the harzardous ingredients in the mix they have mantioned CAS numbers for DMSO and Tween20. I dont know the exact concentrations in the comercial preparations but upto 10% DMSO has been used in regular PCRs to increase the product yield and efficeincy.

In the paper above, I read that SYBR green dye should stable be in 10mM Tris pH 7.5 and frozen in small aliquots.

Cheers

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i our lab we developed and standardise a homemade sybr green mix. its efficency is comparable with the commersiable sybr green mix. But need few changes according to your experimental need.

i am attached the mix...

if any body need further clarification

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Here's a short tech report from Biotechniques: Hope it helps. I'm trying to move from Taqman to Sybr Green I since it's much cheaper, and my PCR is optimized.

http://www.biotechniques.com/default.asp?p...;issue=4/1/2002

BioTechniques vol 32, No.4 (April 2002) Its available in the archives online for free.

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hi,thank u very much.in our lab,we used the homemade sybr green I in real-time PCR to cut the costs ,we just diluted the dye in TE to 10X for stock in -20C ,when we did qPCR, we just diluted the stock solution to 1X in an usual PCR mixture( including template,buffer,primers,taq polymerase,dNTPs),which seemed not to be precise now. Thank you for your composition and i will try that later,but i found there were two tables in your attachment,could you tell me the difference between them?are they used in different pcr methods?and as to the composition,what is "Flourescien",did u mean fluorescence?


best regards

Emily

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