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direct transfection vs. retroviral transfection - (Jan/23/2006 )

Hi all

I'm new in rnai...
and I am wondering what is the different if we transfect the rnai-vector directly to the target cells vs. transfect rnai-vector to 293T cells then use the lentiviral supernatants to infect the target cells (jurkat)??

which one is better?

I'm really confused

thx in advance....

-verencia-

The retrovirus will stably integrate into the DNA of the cell, so expression will be more stable than by transfection
Also,helps for cells that are difficult to transfect.

-tap14-

(1) The efficiency of lentivirus infection will be much higher than plasmid DNA transfection e.g. the number of your target gene that every cell uptake;
(2) also, the integration efficiency into host chromosome (by lentiviral LTR) will be higher;
(3) Broader cell types - lentivirus can be apply to mitotic as well as post-mitotic cells with high gene expression while some hard-to-transfect cell lines will be resistant to conventional DNA transfection method.

-postdoc2130-