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Mitochondria isolation - (Jan/23/2006 )

My questions:

1. Which method can be used for getting high purity of mitochondria from cells beside the kit.
2. which marker can be choosed to check the purity of mitochondria.


My suggestions:

1. Differential centrifugation (old school...)
2. DiOC6(3) at low contration or MitoTracker, by flow cytometry (quantitative)



1.percoll desity gradient centrifuge
2.for integaty, the rate of marker enzyme in mitochondrial matrix under with and without detergent. for purity,the rate of the maker enzyme of mitochondria, cyctol, ER and peroxide.