southern - (Oct/26/2001 )
Hello,
I'am doing to do a southern blotting and i have forgotten to crosslink the DNA on membrane before the prehybridisation step.I would like to know if DNA is always on membrane without crosslinking.Thanksmichel
-ragno-
There will be some DNA but don't expect distinct bands when you probe
-rhodadg-
It depends on the transfer method and the type of membrane. If you did alkali blotting onto N plus membrane it is not necessary to crosslink your DNA because it is covalently linked.
-jbak-