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Southern Blot Backgroundproblems - (Jan/06/2006 )

Hey all,
i did a southern blot followed by Dig-labelling and got a huge background. my supervisor told me to prolong the blocking time and to add 0,1% Tween 20.
Have you ever tried??
Need some help
Thanks smile.gif


Even though the labelling method is different (I use biotinylation for my labelled oligos) most principles should be the same...I would try this link:

go to troubleshooting pages toward the back

good luck! there are many many potential reasons for high background; I hope you can find yours easily