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Acetyl-CoA acetyltransferase- Activity study protocol - (Jan/03/2006 )

Hello Friends,

If anyone working on the Acetyl-CoA acetyltransferase gene from Clostridium or any other bacterial source , could you please share me the activity study procedure.I am looking for a standard protocl to measure the activity in wild and recombinant bacteria.Thanks in advance.


Saikat Chakraborty
Mie University


Can you mix your enzyme with some free cholesterol and 14C oleoyl-CoA let it incubate at 37C for 10-20 minutes, extract the cholesterol ester that is made, run it up a TLC plate, then scrape and count the cholesterol ester band.