immunostaining - (Dec/09/2005 )
ask for help!
I meet some trouble in whole mount staining of mouse ear to detect blood vessel and lymphatic vessel. First, skin, especially, hair follicle present very strong autofluorescence and difficult to get low background photo. Second, the cartilage in ear is difficult for sample permeabilization. Is there any enzyme specific to digest cartilage?
I meet some trouble in whole mount staining of mouse ear to detect blood vessel and lymphatic vessel. First, skin, especially, hair follicle present very strong autofluorescence and difficult to get low background photo. Second, the cartilage in ear is difficult for sample permeabilization. Is there any enzyme specific to digest cartilage?
There enzymes that digest cartilage...Whether they are commercially available or even able to do what you need them for is unknown to me however.
I know a type of Sudan black is used for getting rid autofluor. in brain tissue.
I am not sure about your samples however. Maybe Google it perhaps. Sometimes I have found some answers via Googling up what I am looking for as opposed to searching PubMed.
There enzymes that digest cartilage...Whether they are commercially available or even able to do what you need them for is unknown to me however.
I know a type of Sudan black is used for getting rid autofluor. in brain tissue.
I am not sure about your samples however. Maybe Google it perhaps. Sometimes I have found some answers via Googling up what I am looking for as opposed to searching PubMed.
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Thank you very much for your advice for getting rid autofluor! I will try this method and later I will tell you my results.