how to calculate the percentage of blood vessel area in one certain field? - (Dec/04/2005 )
how to calculate the percentage of blood vessel area in one certain field?
i am now working in angiogenesis and lymphangiogenesis. and i want to find some free software to analyze the percentage of blood vessel area in one certain field. is there anybody good at this field?
-Darcy-
QUOTE (Darcy @ Dec 4 2005, 10:46 PM)
how to calculate the percentage of blood vessel area in one certain field?
i am now working in angiogenesis and lymphangiogenesis. and i want to find some free software to analyze the percentage of blood vessel area in one certain field. is there anybody good at this field?
i am now working in angiogenesis and lymphangiogenesis. and i want to find some free software to analyze the percentage of blood vessel area in one certain field. is there anybody good at this field?
I'm not sure about any free software out there that will do what you need, but there are programs that will do these sorts of calculations. They can cost a fair amount, but you can sometimes obtain "demo" versions from companies or the internet, and some give steep discounts to academic labs.
ImagePro is one example. This program will determine how many pixels are above/below a certain threshold value, and count them for you. So, if all of your blood vessels are darker than background, ImagePro can find and count all the dark pixels, and then you can simply compare that number to how many pixels are in the whole image.
You might be able to do something with Photoshop that is similar (or any program that will find edges or thresholds), but I have not done so personally.
Good luck.
-jreastham4444-
imagej from NIH is free and good
-nexuson-
QUOTE (jreastham4444 @ Dec 5 2005, 05:32 PM)
QUOTE (Darcy @ Dec 4 2005, 10:46 PM)
how to calculate the percentage of blood vessel area in one certain field?
i am now working in angiogenesis and lymphangiogenesis. and i want to find some free software to analyze the percentage of blood vessel area in one certain field. is there anybody good at this field?
I'm not sure about any free software out there that will do what you need, but there are programs that will do these sorts of calculations. They can cost a fair amount, but you can sometimes obtain "demo" versions from companies or the internet, and some give steep discounts to academic labs.
ImagePro is one example. This program will determine how many pixels are above/below a certain threshold value, and count them for you. So, if all of your blood vessels are darker than background, ImagePro can find and count all the dark pixels, and then you can simply compare that number to how many pixels are in the whole image.
You might be able to do something with Photoshop that is similar (or any program that will find edges or thresholds), but I have not done so personally.
Good luck.
Thanks for your advice! I will looking for imagepro.
-Darcy-