chemotaxis study - (Dec/02/2005 )

Dear All,
I wish to study the chemoattractant property of a glycolipid (soluble in organic solvents like chloroform, petroleum ether etc). But we cannot use the Boyden's chamber method as the lipid is insoluble in water based medium. Can any body suggest an alternate method to do the expmt.
Thanking you,
Sneha

hi sneha,
i am not sure whether i can help you completely or not.
other than boyden chamber systems, you can find transwell systems for migration where they use 12well or 24 well plate format in whihc upper chamber will have membrane with different pore sizes.
other commercially availble system is disposable migration chambers. i think you can work with these may be those are bit expensive.
I have not used personnelly but red about these systems. i think you can find information about transwell system and disposable systmes in net.

gud luck