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CsCl gradient - (Nov/23/2005 )


I need a method to purify mtDNA with CsCl gradient from Phytophthora.
Can anyone share his/her method with me?

Also, can anyone give me advice on the most important technical points how to set up the CsCl gradent and how to recover mtDNA band?
I am thinking of those things that are not in the books.

Thanks a lot in advance.



I am isolating mitochondria from different mouse tissues using percoll gradient. it is working fine for me, with also the intact membrane potential of the mito.
the protocol is pretty simple, one has to take care of only the centrifugation speed. i have attached the protocol. You can go through the protocol and then if any questions i brief you about that