nuclear extracts - (Nov/22/2005 )
Hello everyone,
I intend to prepare nuclear extracts from frozen brain tissues. do I have a chance at all? I mean, is freezing them interfere significantly with compartment seperation? do you have a recommendation for a good kit for such purpose? any recommendation for such kit for nuclear extractions from cells?
Many thanks
Shani
-shanib-
check out Active Motif..
although the freezing might interfere with the separation of the different fractions
-Pria-
hi
good check is to analyse briefly two specific proteins :
For nuclear : p53, Histone H1, or myc
For cytoplasmic fraction GAPDH or tubulin will work well
-fred_33-