nuclear extracts - (Nov/22/2005 )
I intend to prepare nuclear extracts from frozen brain tissues. do I have a chance at all? I mean, is freezing them interfere significantly with compartment seperation? do you have a recommendation for a good kit for such purpose? any recommendation for such kit for nuclear extractions from cells?
check out Active Motif..
although the freezing might interfere with the separation of the different fractions
good check is to analyse briefly two specific proteins :
For nuclear : p53, Histone H1, or myc
For cytoplasmic fraction GAPDH or tubulin will work well