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Slow freezing and thawing of FCS - (Nov/21/2005 )

What is the effect of multiple freezing and thawing on the Foetal Calf serum properties' cell culture nutrient??
any ideas...anybody????

-anjali-

One theory is that the growth factors and other important nutrients within the serum will degrade faster if the serum is freeze-thawed regularly. I would recommend derosting the serum overnight at 4˚C, heat-inactivate if required (56˚C 20 min) and then aliquot into 50 ml lots and store frozen. When required, defrost the alquot, dilute in the media bottle and use as required.

Hope this helps,

AussieUSA.

-AussieUSA-